Last data update: 24 January 2024 16:39 CET
Plasmid name: pET3E-His-AU1-hUb-K33 (LMBP 5910)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p5910.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human ubiquitin B cDNA (UBB, Ub, HEL-S-50, GeneID 7314); K33 mutated mature sequence Histidine tag (His-tag); N-terminal AU1-tag; N-terminal |
Promoter: | Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
Terminator: | Phage T7 gene 10 terminator (T7g10) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pET3E |
Further information: | The human ubiquitin K33 cDNA only encodes the wild type lysine number 33; the other six lysine codons are mutated to arginine codons. This plasmid contains the T7 gene 10 promoter whose transcription is initiated by T7 RNA polymerase, making the vector useful for in vitro preparation of single-stranded RNAs from cloned human UB gene. |
EMBL Accession number: | BT020104, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 06/03/2009 |
Sequence detail: | Schematic representation of the human ubiquitin K33 mutant: 6 11 27 29 33 48 63 wild type: 5' --K--K--K--K--K--K--K-- 3' K33 mutant: 5' --R--R--R--R--K--R--R-- 3' |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr K.-L. Guan(1). It was received from Dr. E. Tang(1). (1) Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan, USA |
Plasmid reference: | - |
Related plasmid reference: | Tang et al., J. Biol. Chem. 278 (2003), 37297-37305 [PMID: 12867425] |
Restricted distribution: | - BCCM/MTA adapted by Michigan University |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET3E-His-AU1-hUb-K33 (LMBP 5910) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K.-L. Guan. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.