Last data update: 24 January 2024 16:39 CET
Plasmid name: pGAD-mCASP-2 (LMBP 3850)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3850.gb
(View with Genome Compiler) p3850.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae GAL4 activation domain (GALad) Mouse cysteinyl aspartate specific proteinase 2 cDNA (caspase-2, CASP-2, Casp2, NEDD2, ICH1) |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Ampicillin (amp) LEU2; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-) |
| Parental clone: | pGAD424 |
| Further information: | The plasmid was constructed as follows: full-length mouse caspase-2 cDNA was isolated from a L929r2 cDNA library and inserted into the EcoRI site of pGAD424. The orientation of the inserted fragment appeared to be antisense relative to the ADH1 promoter. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727272.1. The nucleotide sequence of the mCASP-2 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number BC034262.1, except for some 3' UTR nucleotides which correspond to the genomic sequence. These differences are described in the EMBL record. Other name of the plasmid is pgadF72. |
| EMBL Accession number: | BC034262.1, view at EMBL, GenBank, DDBJ LT727272.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 28/04/1998 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BanI, EcoRI and SphI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Vandenabeele(1)(2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Van de Craen et al., FEBS Lett. 403 (1997), 61-69 [PMID: 9038361] |
| Related plasmid reference: | Van de Craen et al., Eur. J. Immunol. 27 (1997), 1296-1299 [PMID: 9174624] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGAD-mCASP-2 (LMBP 3850) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Vandenabeele and was published in Van de Craen et al., 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.