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GeneCorner plasmid details

Last data update: 20 September 2021 09:16 CEST

Plasmid name: pGAD-mCASP-6 (LMBP 3849)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p3849.gb (View with Genome Compiler)
p3849.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Saccharomyces cerevisiae GAL4 activation domain (GALad)
Mouse cysteinyl aspartate specific proteinase 6 cDNA (caspase-6, CASP-6, Mch2, Casp6)
Promoter: Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1)
Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator
Selection marker: Ampicillin (amp)
LEU2; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB)
Host range: Escherichia coli
Saccharomyces cerevisiae; leu2(-)
Parental clone: pGAD424
Further information: The plasmid was constructed as follows: full-length mouse caspase-6 cDNA was isolated from a L929r2 cDNA library and inserted into the EcoRI site of pGAD424. The orientation of the inserted fragment appeared to be antisense relative to the ADH1 promoter.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727271.1.
Other name of the plasmid is pgadE16.
EMBL Accession number: LT727271.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 28/04/1998
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, NsiI and XmnI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr P. Vandenabeele(1)(2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Van de Craen et al., FEBS Lett. 403 (1997), 61-69 [PMID: 9038361]
Related plasmid reference: Van de Craen et al., Eur. J. Immunol. 27 (1997), 1296-1299 [PMID: 9174624]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pGAD-mCASP-6 (LMBP 3849) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Vandenabeele and was published in Van de Craen et al., 1997.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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