Last data update: 30 October 2020 04:11 CET
Plasmid name: pGB-11-12-ccdB (LMBP 12035)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
|Cloned DNA:||GoldenBac entry module
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Strep-tag III (Twin-Strep-tag)
Human rhinovirus 3C protease cleavage site (PreScission site, PRS)
Histidine tag (His-tag)
|Promoter:||Autographa californica nuclear polyhedrosis virus (AcNPV) polyhedrin promoter (PH)
Escherichia coli class 1 integron integrase promoter (intI1)
Escherichia coli plasmid R388 class 1 integron Pc promoter (PcS)
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Gentamicin (Gm)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use a ccdB-resistant strain for propagation
|Further information:||The plasmid was constructed by cloning a cassette consisting of BsaI-pAcNPV-PH-Strep-Prescission-ccdB-His-SV40-BsaI into the pGB vector. This pGB vector is based on pACEBac1 from the MultiBac system.
This plasmid is an entry cloning vector for single gene expression of recombinant proteins in insect cells using a Bac-to-Bac baculoviral expression system (GoldenBac). With 16 different overhangs, the system allows for co-expression of up to 15 genes after assembly into a destination vector.
GoldenBac is a modular cloning system designed for insect cells which makes use of a single type IIS restriction endonuclease (BsaI).
The BsaI cassette is flanked by Tn7 transposon sequences.
Other name of the plasmid is pGB-11;12_ccdB.
|EMBL Accession number:||-|
|Latest sequence update:||29/11/2019|
Nucleotide sequence of the BsaI cassette: GGTCTCAAGAA...-- pPH ->....-- SV40 polyA ->...GTTGTGAGACC BsaI ^^^^ **** BsaI ^^^^: BsaI overhang 11 ****: BsaI overhang 12
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Dr D. Soroldoni(1). It was constructed by Dr J. Neuhold(1).
(1) Vienna Biocenter Core Facilities GmbH, Vienna, Austria
|Plasmid reference:||Neuhold et al., BMC Biotechnology 20 (2020), 26 [PMID: 32398045] [DOI: 10.1186/s12896-020-00616-z]
|Related plasmid reference:||Lampropoulos et al., PLoS ONE 8 (2013), e83043 [PMID: 24376629] [DOI: 10.1371/journal.pone.0083043]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12xB DB3.1|
|Related host reference:||Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
|Cultivation medium:||LB-Lennox + gentamicin (12,5 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pGB-11-12-ccdB (LMBP 12035) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D. Soroldoni and was published in Neuhold et al., 2020.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.