Last data update: 30 October 2020 04:11 CET
Plasmid name: pGB-dummy-06-16 (LMBP 12015)
|Price category:||Cat. 2 (cf. price list)|
|Status:||GeneCorner core plasmid|
|GeneCorner sequence:||p12015.gb (View with Genome Compiler)|
|Cloned DNA:||GoldenBac stuffer fragment
|Promoter:||Phage T7 gene 10 promoter (T7g10)
Phage SP6 promoter
Escherichia coli lac operon promoter
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli
|Further information:||The plasmid was constructed by cloning a stuffer fragment with variable flanking ends into the BsaI-opened pGG vector. This pGG vector is based on pUC19 that was adapted by Lampropoulos et al. for the GreenGate assembly system.
The plasmid is intended to encompass unused positions in co-expression assemblies of the GoldenBac system.
GoldenBac is a modular cloning system designed for insect cells which makes use of a single type IIS restriction endonuclease (BsaI).
Other name of the plasmid is pGB-dummy-06;16.
|EMBL Accession number:||-|
|Latest sequence update:||29/11/2019|
Nucleotide sequence of the BsaI cassette: GGTCTCAACTAGGCTGAGTAGGCAAGATGTTCTGGTATTGAGACC BsaI ^^^^<-- dummy sequence --->**** BsaI ^^^^: BsaI overhang 06 ****: BsaI overhang 16
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: DraI/PvuII, EcoRI/NdeI and HindIII.
The region of the GoldenBac cassette was sequenced at GeneCorner: downstream of the pMB1 ori to the 5' end of the ampicillin resistance gene.
|History of deposit:||This plasmid was deposited by Dr D. Soroldoni(1). It was constructed by Dr J. Neuhold(1).
(1) Vienna Biocenter Core Facilities GmbH, Vienna, Austria
|Plasmid reference:||Neuhold et al., BMC Biotechnology 20 (2020), 26 [PMID: 32398045] [DOI: 10.1186/s12896-020-00616-z]
|Related plasmid reference:||Lampropoulos et al., PLoS ONE 8 (2013), e83043 [PMID: 24376629] [DOI: 10.1371/journal.pone.0083043]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 DH5αT1R|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pGB-dummy-06-16 (LMBP 12015) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D. Soroldoni and was published in Neuhold et al., 2020.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.