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GeneCorner plasmid details

Last data update: 18 January 2021 04:24 CET

Plasmid name: pGEME6Hf1 (LMBP 1978)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1978.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; truncated heavy chain of the Fab fragment E6F1 (E6Hf1)
Promoter: Phage SP6 promoter
Phage T7 gene 10 promoter (T7g10)
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pGEME6H
Further information: The plasmid was constructed as follows: 1) pGEME6H was cleaved with ApaI (10th nucleotide of the hinge sequence) and blunted (Klenow fragment); 2) an XbaI linker ('TCTAGA') was added by ligation, resulting in the vector pGEME6H3 which contains a termination codon preceding the first Cys codon of the hinge sequence; 3) the XbaI fragment from pGEME6H3 was subsequently deleted by an XbaI digestion.
EMBL Accession number: -
Latest sequence update: 19/04/1990
Sequence detail:
Nucleotide sequence around the newly created termination codon behind the CH1 region:

                       *
5' CTT.GAG.CCC.AGC.GTC.TAG AGGATCCCC 3'
                    XbaI

*: Termination codon behind the CH1 region.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: De Sutter et al., Gene 113 (1992), 223-230 [PMID: 1572543]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pGEME6Hf1 (LMBP 1978) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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