Last data update: 22 May 2023 13:52 CEST
Plasmid name: pGFP-N-GW (LMBP 8367)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p8367.gb
(View with Genome Compiler) p8367.txt p8367.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene) Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP), N-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli lac operon promoter; mutant (lacUV5) Escherichia coli β-lactamase promoter (amp) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli; use a ccdB-resistant strain for propagation Mammalian cells; SV40 permissive cells |
| Parental clone: | pEGFP-C1 |
| Further information: | This is a Gateway destination vector meant for N-terminal fusion of the EGFP reporter gene. This Gateway destination vector is compatible with the human orfeome library which is also available at BCCM/GeneCorner. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726799.1. The nucleotide sequence of EGFP corresponds with the EMBL Nucleotide Sequence Database accession number U55761.1. |
| EMBL Accession number: | U55761.1, view at EMBL, GenBank, DDBJ LT726799.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/06/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: NheI/XhoI, NcoI, NotI and PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr S. Goethals(1) and Prof. Dr V. Timmerman(1). It was constructed by Dr L. de Almeida Souza(2). (1) Department of Molecular Genetics, VIB, Antwerp, Belgium (2) Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge, UK |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB DB3.1 |
| Host reference: | - |
| Related host reference: | Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324] |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) + kanamycin (50 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with kanamycin. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGFP-N-GW (LMBP 8367) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Goethals and Prof. Dr V. Timmerman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.