Last data update: 24 January 2024 16:39 CET
Plasmid name: pLT10T3A20 (LMBP 2862)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2862.gb
(View with Genome Compiler) p2862.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human TNF alpha induced protein 3 cDNA (TNFAIP3, A20, OTUD7C, GeneID 7128) |
| Promoter: | Phage λ major leftward promoter (λ PL) Phage T7 gene 10 promoter (T7g10) Phage T3 promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage fd terminator Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pLT10T3 |
| Further information: | The plasmid was constructed as follows: the coding sequence of the human zinc finger protein A20 cDNA (hA20) was isolated from MCF7 human breast carcinoma cells by the use of primers, and inserted as a NdeI/XhoI fragment between the NdeI and XhoI sites of the polylinker of pLT10T3. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727550.1. The nucleotide sequence of the hA20 coding sequence corresponds with the Genbank accession number M59465.1. |
| EMBL Accession number: | M59465.1, view at EMBL, GenBank, DDBJ LT727550.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/02/2004 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI and HindII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium It was constructed by S. Zini. |
| Plasmid reference: | De Valck et al., FEBS Lett. 384 (1996), 61-64 [PMID: 8797804] |
| Related plasmid reference: | Coornaert et al., Nat. Immunol. 9 (2008), 263-271 [PMID: 18223652] [DOI: 10.1038/ni1561] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLT10T3A20 (LMBP 2862) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in De Valck et al., 1996. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.