Last data update: 06 May 2021 08:34 CEST
Plasmid name: pMa58 (LMBP 1461)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Terminator:||Phage fd terminator|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Phage f1 origin
|Host range:||Escherichia coli|
|Further information:||The plasmid was designed for site-specific mutagenesis where the mutated sequence can be physically linked with a selectable marker (chloramphenicol).
The plasmid provides resistance to ampicillin, but is sensitive to chloramphenicol.
More than one mutagenesis round is possible via switching from ampicillin resistance to chloramphenicol resistance.
pMa58 contains the replication origin of the single-stranded DNA phage f1 (clockwise orientated), so that it can be rapidly switched between the plasmid and the phage mode (ssDNA) of replication. The latter requires infection with a helper phage, e.g. M13KO7.
After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6).
Name mentioned in Stanssens et al. (1989) is pMa5-8.
|EMBL Accession number:||-|
|Latest sequence update:||19/05/1989|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BglI/NgoMIV, EcoRI, HindII, PstI and ScaI. The plasmid seems to be approx. 200 bp larger as compared to the compiled nucleotide sequence.|
|History of deposit:||This plasmid was deposited by Dr P. Stanssens(1).
(1) Corvas International N.V., Ghent, Belgium
|Plasmid reference:||Stanssens et al., Nucleic Acids Res. 17 (1989), 4441-4454 [PMID: 2501754]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061(λ)|
|Host reference:||Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
|Related host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pMa58 (LMBP 1461) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Stanssens and was published in Stanssens et al., 1989.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.