Last data update: 24 January 2024 16:39 CET
Plasmid name: pMc58 (LMBP 1462)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pmc58.gb
(View with Genome Compiler) pmc58.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Phage fd terminator |
| Selection marker: | Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pMac5-8 |
| Further information: | The phasmid was designed for site-specific mutagenesis where the mutated sequence can be physically linked with a selectable marker (amp). The phasmid provides resistance to chloramphenicol, but is sensitive to ampicillin. More than 1 mutagenesis round is possible via switching from chloramphenicol resistance to ampicillin resistance. pMc58 contains the replication origin of the single-stranded DNA phage f1 (clockwise orientated), so that it can be rapidly switched between the plasmid and the phage mode (ssDNA) of replication. The latter requires infection with a helper phage, e.g. M13KO7. After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727577.1. |
| EMBL Accession number: | LT727577.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 22/05/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI, PstI and SspI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. Stanssens(1). (1) Corvas International N.V., Ghent, Belgium |
| Plasmid reference: | Stanssens et al., Nucleic Acids Res. 17 (1989), 4441-4454 [PMID: 2501754] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + chloramphenicol (25 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMc58 (LMBP 1462) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Stanssens and was published in Stanssens et al., 1989. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.