Last data update: 24 January 2024 16:39 CET
Plasmid name: pMcTrpMTNFm29S32W (LMBP 2656)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p2656.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Mouse tumor necrosis factor cDNA (Tnf, TNF); L29S-R32W mutant |
Promoter: | Escherichia coli tryptophan operon promoter (trp) |
Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp) |
Terminator: | Phage fd terminator |
Selection marker: | Chloramphenicol (cam) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
Host range: | Escherichia coli |
Parental clone: | pMaTrpMTNF |
Further information: | This plasmid was constructed by site-specific mutagenesis, where the nucleotide sequences of amino acids 29 and 32 of the mature mouse TNF (CTG, coding for Leu and CGC, coding for Arg) were converted to TCG (coding for Ser) and TGG (coding for Trp). After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6). |
EMBL Accession number: | M11731, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 17/01/1993 |
Sequence detail: | RBS trp | mature mouse TNF | 1 2 10 5'... GTATCGATTCC.ATG.GTA.AGA.TCA.AGT.AGT.CAA.AAT.TCG.AGT.GAC.AAG.CCT.GTA.GCC.CAC.GTC.GTA. NcoI * * ** ** TaqI Val 20 29 32 .GCA.AAC.CAC.CAA.GTG.GAG.GAG.CAG.CTG.GAG.TGG.TCG.AGC.CAG.TGG.GCC.AAC ...3' ** ** TaqI HaeIII Ser Trp |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). The plasmid was constructed by Dr Xaveer Van Ostade. (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | Van Ostade et al., Eur. J. Biochem. 220 (1994), 771-779 [PMID: 8143731] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 SURE |
Host reference: | Greener, Strategies 3 (1990), 5-6 |
Cultivation medium: | LB-Lennox + chloramphenicol (25 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMcTrpMTNFm29S32W (LMBP 2656) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Van Ostade et al., 1994. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.