Last data update: 18 January 2021 04:24 CET
Plasmid name: pOMMB (LMBP 2079)
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
Human immunoglobulin γ3 cDNA (hIG3); heavy chain encoding the human γ heavy chain disease (HCD) OMMB protein|
|Selection marker:||Tetracycline (tet)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Parental clone:||pBR322; pOMMA|
|Further information:||The leader fragment (Lf) of the pOMMB clone contains the last 3 codons of the entire leader sequence of pOMMA.
The variable region fragment (VHf) exists of the first 15 codons of the full-length variable region.
The CH1 domain is totally omitted, while the hinge region is complete.
The length of the dG/dC-tail of the pOMMA insert is unknown.
The nucleotide sequence encoding the human γ heavy chain disease (HCD) OMM protein was obtained from Genbank 'Release 55; Locus : HUMIGHAF'. The unknown nucleotides in the Genbank-obtained sequence have been sequenced (Dr R. Schoonjans and K. De Sutter, Department of Biomedical Molecular Biology, Ghent University, Belgium).
|EMBL Accession number:||J00231, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||13/03/1996|
Nucleotide sequence at the start of the pOMMA insert: 5' GGGGG GTC.CTG.TCC.CAG.GTG.CAC.CTG.CAG.GAG.TCG.GGC.CCA.GGA.CTG. * + GGG.AAG.CCT.CCA.GAG.CTC.AAA.ACC. 3' ^ *: Start leader signal fragment (Lf). +: Start variable region fragment (VHf). ^: Start hinge region (H1-4). Punctuation indicates reading frame.
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr J.N. Buxbaum(1).
(1) Irvington House Institute, New York, USA
|Plasmid reference:||Alexander et al., Proc. Natl. Acad. Sci. U.S.A. 79 (1982), 3260-3264 [PMID: 6808505]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + tetracycline (10 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pOMMB (LMBP 2079) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J.N. Buxbaum and was published in Alexander et al., 1982.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.