Last data update: 24 January 2024 16:39 CET
Plasmid name: pOMMB (LMBP 2079)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | pommb.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human immunoglobulin γ3 cDNA (hIG3); heavy chain encoding the human γ heavy chain disease (HCD) OMMB protein |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322; pOMMA |
| Further information: | The leader fragment (Lf) of the pOMMB clone contains the last 3 codons of the entire leader sequence of pOMMA. The variable region fragment (VHf) exists of the first 15 codons of the full-length variable region. The CH1 domain is totally omitted, while the hinge region is complete. The length of the dG/dC-tail of the pOMMA insert is unknown. The nucleotide sequence encoding the human γ heavy chain disease (HCD) OMM protein was obtained from Genbank 'Release 55; Locus : HUMIGHAF'. The unknown nucleotides in the Genbank-obtained sequence have been sequenced (Dr R. Schoonjans and K. De Sutter, Department of Biomedical Molecular Biology, Ghent University, Belgium). |
| EMBL Accession number: | J00231, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 13/03/1996 |
| Sequence detail: | Nucleotide sequence at the start of the pOMMA insert:
5' GGGGG GTC.CTG.TCC.CAG.GTG.CAC.CTG.CAG.GAG.TCG.GGC.CCA.GGA.CTG.
* +
GGG.AAG.CCT.CCA.GAG.CTC.AAA.ACC. 3'
^
*: Start leader signal fragment (Lf).
+: Start variable region fragment (VHf).
^: Start hinge region (H1-4).
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr J.N. Buxbaum(1). (1) Irvington House Institute, New York, USA |
| Plasmid reference: | Alexander et al., Proc. Natl. Acad. Sci. U.S.A. 79 (1982), 3260-3264 [PMID: 6808505] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pOMMB (LMBP 2079) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J.N. Buxbaum and was published in Alexander et al., 1982. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.