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GeneCorner plasmid details

Last data update: 18 January 2021 04:24 CET

Plasmid name: pOMMB (LMBP 2079)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: pommb.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human immunoglobulin γ3 cDNA (hIG3); heavy chain encoding the human γ heavy chain disease (HCD) OMMB protein
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pBR322; pOMMA
Further information: The leader fragment (Lf) of the pOMMB clone contains the last 3 codons of the entire leader sequence of pOMMA.
The variable region fragment (VHf) exists of the first 15 codons of the full-length variable region.
The CH1 domain is totally omitted, while the hinge region is complete.
The length of the dG/dC-tail of the pOMMA insert is unknown.
The nucleotide sequence encoding the human γ heavy chain disease (HCD) OMM protein was obtained from Genbank 'Release 55; Locus : HUMIGHAF'. The unknown nucleotides in the Genbank-obtained sequence have been sequenced (Dr R. Schoonjans and K. De Sutter, Department of Biomedical Molecular Biology, Ghent University, Belgium).
EMBL Accession number: J00231, view at EMBL, GenBank, DDBJ
Latest sequence update: 13/03/1996
Sequence detail:
Nucleotide sequence at the start of the pOMMA insert:


5' GGGGG GTC.CTG.TCC.CAG.GTG.CAC.CTG.CAG.GAG.TCG.GGC.CCA.GGA.CTG.
         *           +


GGG.AAG.CCT.CCA.GAG.CTC.AAA.ACC. 3'
                ^


*: Start leader signal fragment (Lf).
+: Start variable region fragment (VHf).
^: Start hinge region (H1-4).
Punctuation indicates reading frame.
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr J.N. Buxbaum(1).
(1) Irvington House Institute, New York, USA
Plasmid reference: Alexander et al., Proc. Natl. Acad. Sci. U.S.A. 79 (1982), 3260-3264 [PMID: 6808505]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pOMMB (LMBP 2079) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J.N. Buxbaum and was published in Alexander et al., 1982.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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