Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLST10 (LMBP 2044)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | pplst10.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Satellite tobacco necrosis virus (STNV) |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function |
| Parental clone: | pPLc2820; pUR222 |
| Further information: | The plasmid was constructed as follows: the dG/dC-tailed cDNA of STNV was inserted into the unique PstI site of a modified pPLc2820: at the 3' end of the STNV gene, the PstI site is located next to the SalI site and the XbaI site in between is lost. The inserted STNV fragment is complete. Detailed construction information can be found in Van Emmelo et al. (1984), fig. 1 p. 34. Names mentioned in the reference are pCP403 and pSTNV403. Other name of the plasmid is pSTPU1. |
| EMBL Accession number: | - |
| Latest sequence update: | 12/09/1989 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Van Emmelo et al., Virology 136 (1984), 32-40 [PMID: 18639819] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 K514(λ) |
| Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLST10 (LMBP 2044) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Van Emmelo et al., 1984. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.