Last data update: 11 June 2021 14:12 CEST
Plasmid name: pPLSTi953 (LMBP 1818)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
Satellite tobacco necrosis virus (STNV); with insertion|
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function|
|Further information:||This plasmid is derived from pPLST10; after a partial EcoRV digestion (nucleotide position 953 of STNV) the EcoRI-NcoI linker (AGAATTCCCATGGA) was inserted.
Name mentioned in Van Emmelo et al. (1984) is pSTNVdelI.
Other names of the plasmid are pSTPU1L44, pPLSTNVR52 and pSTNVE951.
|EMBL Accession number:||-|
|Latest sequence update:||22/09/1989|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Van Emmelo et al., Virology 136 (1984), 32-40 [PMID: 18639819]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 r-m+ (λ)|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pPLSTi953 (LMBP 1818) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Van Emmelo et al., 1984.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.