Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLSTi953 (LMBP 1818)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p1818.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Satellite tobacco necrosis virus (STNV); with insertion |
Promoter: | Phage λ major leftward promoter (λ PL) |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli; use strains with a cI function |
Parental clone: | pPLST10 |
Further information: | This plasmid is derived from pPLST10; after a partial EcoRV digestion (nucleotide position 953 of STNV) the EcoRI-NcoI linker (AGAATTCCCATGGA) was inserted. Name mentioned in Van Emmelo et al. (1984) is pSTNVdelI. Other names of the plasmid are pSTPU1L44, pPLSTNVR52 and pSTNVE951. |
EMBL Accession number: | - |
Latest sequence update: | 22/09/1989 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | Van Emmelo et al., Virology 136 (1984), 32-40 [PMID: 18639819] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 r-m+ (λ) |
Host reference: | - |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLSTi953 (LMBP 1818) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Van Emmelo et al., 1984. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.