Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc2819 (LMBP 482)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
pplc2819.gb
(View with Genome Compiler) pplc2819.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Phage λ major leftward promoter (λ PL) |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
Parental clone: | pPLc28 |
Further information: | The plasmid is designed for expression of fragments containing a ribosome binding site functional in E. coli. There are two HindIII and three Xbal expression sites in the polylinker downstream from the λ PL promoter. The PstI expression site is not unique: there is another one in the ampicillin resistance gene. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727044.1. Other name of the plasmid is pST2819 . |
EMBL Accession number: | LT727044.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 26/01/1989 |
Sequence detail: | Nucleotide sequence of the polylinker downstream from the PL promoter: 5' GAATTCCGGATCCGGCCAAGCTTGGCTCTAGAGGTCGACCCTCTAGA EcoRI BamHI HindIII XbaI SalI XbaI BspMII GGCTGCAGCCTCTAGAGCCAAGCTT 3' PstI XbaI HindIII |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HaeII, PstI and PvuI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 r-m+ (λ) |
Host reference: | - |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc2819 (LMBP 482) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Remaut et al., 1981. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.