Last data update: 03 December 2021 13:45 CET
Plasmid name: pPLc2819 (LMBP 482)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Further information:||The plasmid is designed for expression of fragments containing a ribosome binding site functional in E. coli.
There are two HindIII and three Xbal expression sites in the polylinker downstream from the λ PL promoter.
The PstI expression site is not unique: there is another one in the ampicillin resistance gene.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727044.1.
Other name of the plasmid is pST2819 .
|EMBL Accession number:||LT727044.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||26/01/1989|
Nucleotide sequence of the polylinker downstream from the PL promoter: 5' GAATTCCGGATCCGGCCAAGCTTGGCTCTAGAGGTCGACCCTCTAGA EcoRI BamHI HindIII XbaI SalI XbaI BspMII GGCTGCAGCCTCTAGAGCCAAGCTT 3' PstI XbaI HindIII
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: HaeII, PstI and PvuI.|
|History of deposit:||This plasmid was deposited by Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Plasmid reference:||Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 r-m+ (λ)|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pPLc2819 (LMBP 482) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Remaut et al., 1981.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.