Last data update: 15 October 2021 09:33 CEST
Plasmid name: pPLc2820 (LMBP 1442)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Promoter:||Phage λ major leftward promoter (λ PL)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains with a cI function, cIts for PL controlled expression|
|Parental clone:||pPLc2819; pUC8|
|Further information:||The plasmid is designed for expression of fragments containing a ribosome binding site functional in E. coli.
pPLc2820 differs from pPLc2819 in the orientation of the polylinker downstream from the λ-PL promoter and having a unique PstI expression site.
pPLc2820 has no PstI site in the ampicillin resistance gene.
The HindIII and XbaI expression sites are not unique.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727574.1.
|EMBL Accession number:||LT727574.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||01/02/1989|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI, HindII, MspA1I and RsaI.|
|History of deposit:||This plasmid was deposited by R. Leemans(1) and Prof. Dr E. Remaut(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061(λ)|
|Host reference:||Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329]
|Related host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pPLc2820 (LMBP 1442) is available at BCCM/GeneCorner. This plasmid was deposited by R. Leemans and Prof. Dr E. Remaut.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.