Last data update: 24 January 2024 16:39 CET
Plasmid name: pRM1 (LMBP 4345)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4345.gb
(View with Genome Compiler) p4345.txt |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Candida albicans URA3; auxotrophic Candida albicans LEU2; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Candida albicans autonomously replicating sequence ARS2 Candida albicans autonomously replicating sequence ARS3 |
| Host range: | Escherichia coli Candida albicans; leu2(-), ura3(-) |
| Parental clone: | pRC2312; pEH7 |
| Further information: | The construction of this plasmid is described by Pla et al. (1995). pRM1 is a non-integrative, double-ARS shuttle vector for Candida albicans strains. The presence of both C. albicans autonomously replicating sequences ARS2 and ARS3 allows direct recovery in Escherichia coli of non-multimerized plasmids from C. albicans transformants without any intermediate step in Saccharomyces cerevisiae. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727194.1. The nucleotide sequence of the Candida URA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198.1. |
| EMBL Accession number: | X14198.1, view at EMBL, GenBank, DDBJ LT727194.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 16/01/2002 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BseRI, DrdI, EcoRI, HindIII, NcoI, NotI, SmaI and SphI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Pla(1). (1) Department Microbiologia II, Facultad de Farmacia, Universidad Complutense de Madrid, Spain |
| Plasmid reference: | Pla et al., Gene 165 (1995), 115-120 [PMID: 7489899] |
| Related plasmid reference: | Herreros et al., Mol. Microbiol. 6 (1992), 3567-3574 [PMID: 1474898] Kelly et al., Mol. Gen. Genet. 214 (1988), 24-31 [PMID: 2976118] Cannon et al., Mol. Gen. Genet. 221 (1990), 210-218 [PMID: 2196431] Cannon et al., Mol. Gen. Genet. 235 (1992), 453-457 [PMID: 1465116] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pRM1 (LMBP 4345) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Pla and was published in Pla et al., 1995. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.