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GeneCorner plasmid details

Last data update: 18 January 2021 04:24 CET

Plasmid name: pRM1 (LMBP 4345)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4345.gb (View with Genome Compiler)
p4345.txt
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Candida albicans URA3; auxotrophic
Candida albicans LEU2; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Candida albicans autonomously replicating sequence ARS2
Candida albicans autonomously replicating sequence ARS3
Host range: Escherichia coli
Candida albicans; leu2(-), ura3(-)
Parental clone: pRC2312; pEH7
Further information: The construction of this plasmid is described by Pla et al. (1995).
pRM1 is a non-integrative, double-ARS shuttle vector for Candida albicans strains.
The presence of both C. albicans autonomously replicating sequences ARS2 and ARS3 allows direct recovery in Escherichia coli of non-multimerized plasmids from C. albicans transformants without any intermediate step in Saccharomyces cerevisiae.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727194.1.
The nucleotide sequence of the Candida URA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number X14198.1.
EMBL Accession number: X14198.1, view at EMBL, GenBank, DDBJ
LT727194.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 16/01/2002
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, BseRI, DrdI, EcoRI, HindIII, NcoI, NotI, SmaI and SphI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Pla(1).
(1) Department Microbiologia II, Facultad de Farmacia, Universidad Complutense de Madrid, Spain
Plasmid reference: Pla et al., Gene 165 (1995), 115-120 [PMID: 7489899]
Related plasmid reference: Cannon et al., Mol. Gen. Genet. 235 (1992), 453-457 [PMID: 1465116]
Cannon et al., Mol. Gen. Genet. 221 (1990), 210-218 [PMID: 2196431]
Kelly et al., Mol. Gen. Genet. 214 (1988), 24-31 [PMID: 2976118]
Herreros et al., Mol. Microbiol. 6 (1992), 3567-3574 [PMID: 1474898]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pRM1 (LMBP 4345) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Pla and was published in Pla et al., 1995.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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