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GeneCorner plasmid details

Last data update: 26 November 2020 04:30 CET

Plasmid name: pRS414 (LMBP 4058)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4058.gb (View with Genome Compiler)
p4058.txt
p4058.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli lac Z gene (lacZ); fragment, incl. α part
Promoter: Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: -
Selection marker: Ampicillin (amp)
Saccharomyces cerevisiae TRP1; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4
Host range: Escherichia coli
Saccharomyces cerevisiae; trp1(-)
Parental clone: pRS404
Further information: This is a single-copy Yeast Centromere Plasmid (YCp), designed for efficient manipulation of DNA in S. cerevisiae.
The plasmid contains a multi-cloning site (MCS) in the LacZ α-fragment for insert detection using α-complementation in E. coli.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number U03448.1.
EMBL Accession number: U03448.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 13/03/2000
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglI/EcoRI, EcoRV, HincII and HindIII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr Philip Hieter(1).
(1) Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Plasmid reference: Sikorski et al., Genetics 122 (1989), 19-27 [PMID: 2659436]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pRS414 (LMBP 4058) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Philip Hieter and was published in Sikorski et al., 1989.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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