GREAT AT SMALL THINGS

0

GeneCorner plasmid details

Last data update: 26 November 2020 04:30 CET

Plasmid name: pRS424 (LMBP 4062)

Add to cart

New search

Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: prs424.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli lac Z gene (lacZ); fragment, incl. α part
Promoter: Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator
Selection marker: Ampicillin (amp)
Saccharomyces cerevisiae TRP1; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB)
Host range: Escherichia coli
Saccharomyces cerevisiae; trp1(-)
Parental clone: pBluescriptIIKS+
Further information: This is a high-copy Yeast Episomal Plasmid (YEp), designed for efficient manipulation of DNA in S. cerevisiae.
The plasmid contains a multi-cloning site (MCS) in the LacZ α-fragment for insert detection using α-complementation in E. coli.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number U03453.1.
EMBL Accession number: U03453.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 13/03/2000
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr Philip Hieter(1).
(1) Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Plasmid reference: Christianson et al., Gene 110 (1992), 119-122 [PMID: 1544568]
Related plasmid reference: Sikorski et al., Genetics 122 (1989), 19-27 [PMID: 2659436]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pRS424 (LMBP 4062) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Philip Hieter and was published in Christianson et al., 1992.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search