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GeneCorner plasmid details

Last data update: 26 November 2020 04:30 CET

Plasmid name: pSCGAL1mBAX-L (LMBP 4200)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p4200.gb (View with Genome Compiler)
p4200.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse Bcl2-associated X cDNA (Bax, BAX); α variant
Promoter: Saccharomyces cerevisiae galactokinase promoter (GAL1)
Ribosome
binding site:
-
Terminator: Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator
Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1)
Selection marker: Ampicillin (amp)
LEU2; auxotrophic
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Saccharomyces cerevisiae; leu2(-), integrative
Parental clone: pSCGAL1-L; pUC19muBAX
Further information: The plasmid was constructed by inserting the XbaI-HindIII fragment from pUC19muBAX, containing the α variant of the mouse Bcl2-associated X cDNA (mBAX) into the XbaI-HindIII opened pSCGAL1-L plasmid.
pSCGAL1mBAX-L is an integrative expression plasmid for the mouse Bax-α protein in Saccharomyces cerevisiae. After BstEII linearisation in its LEU2 element, single-copy genomical integration leads to stable expression.
The nucleotide sequence of the mBAX cDNA corresponds with the EMBL Nucleotide Sequence Database accession number L22472.1.
Other names of the plasmid are YIpUTL-muBax, YIpUTLB and pSCGALmBAX1.
EMBL Accession number: L22472.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 11/09/2000
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: AgeI/XmnI, BamHI, BglI/SphI, BstEII/XhoI, HindIII/XbaI and MluI.

The XmnI site at position 4243 could not be experimentally confirmed.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr R. Contreras(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pSCGAL1mBAX-L (LMBP 4200) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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