GREAT AT SMALL THINGS

0

GeneCorner plasmid details

Last data update: 21 October 2021 10:13 CEST

Plasmid name: pSV/TXBP15-1-AS (LMBP 3515)

Add to cart

New search Print data sheet
Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p3515.gb (View with Genome Compiler)
p3515.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human T-cell leukemia virus type I (HTLV-1) Tax 1 binding protein 1 cDNA (TXBP15-1; TXBP151; TAX1BP1); small form (TAX1BP1-S)
Promoter: Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pM
Further information: This plasmid was constructed by inserting the splice variant of the human Tax1 binding protein 1 cDNA (TAX1BP1-S) as an EcoRI-XhoI fragment between the EcoRI and XhoI sites of pM (Clontech).
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727346.1.
Referring to the pCAGGS-TAX1BP1-S sequence analysis results of the Department of Biomedical Molecular Biology (Ghent University, Belgium), the human TAX1BP1-S coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number U33821.2, except for the nucleotides CG at position 147-148 being substituted by GC, resulting in H49Q and V50L mutations, and nucleotide T at position 1203 being substituted by C, resulting in a silent mutation. These differences were confirmed by Dr K-T. Jeang (NIH, Bethesda, Maryland, USA).
Other names of the plasmid are pMTAXBP151(antisense) and pMTAXBP151(AS)s.
EMBL Accession number: U33821.2, view at EMBL, GenBank, DDBJ
LT727346.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 12/04/2002
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII/XhoI, EcoRI, MspA1I, RsaI and XmnI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr Kuan-Teh Jeang(1).
(1) NIH, Bethesda, Maryland, USA
Plasmid reference: -
Related plasmid reference: Sadowski et al., Nucleic Acids Res. 17 (1989), 7539 [PMID: 2798115]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pSV/TXBP15-1-AS (LMBP 3515) is available at BCCM/GeneCorner. This plasmid was deposited by Dr Kuan-Teh Jeang.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search