Last data update: 21 October 2021 10:13 CEST
Plasmid name: pSV/TXBP15-1-AS (LMBP 3515)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
Human T-cell leukemia virus type I (HTLV-1) Tax 1 binding protein 1 cDNA (TXBP15-1; TXBP151; TAX1BP1); small form (TAX1BP1-S)|
|Promoter:||Simian virus 40 early promoter (SV40 early)|
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Further information:||This plasmid was constructed by inserting the splice variant of the human Tax1 binding protein 1 cDNA (TAX1BP1-S) as an EcoRI-XhoI fragment between the EcoRI and XhoI sites of pM (Clontech).
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727346.1.
Referring to the pCAGGS-TAX1BP1-S sequence analysis results of the Department of Biomedical Molecular Biology (Ghent University, Belgium), the human TAX1BP1-S coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number U33821.2, except for the nucleotides CG at position 147-148 being substituted by GC, resulting in H49Q and V50L mutations, and nucleotide T at position 1203 being substituted by C, resulting in a silent mutation. These differences were confirmed by Dr K-T. Jeang (NIH, Bethesda, Maryland, USA).
Other names of the plasmid are pMTAXBP151(antisense) and pMTAXBP151(AS)s.
|EMBL Accession number:||U33821.2, view at EMBL, GenBank, DDBJ
LT727346.1, view at EMBL, GenBank, DDBJ
|Latest sequence update:||12/04/2002|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII/XhoI, EcoRI, MspA1I, RsaI and XmnI.|
|History of deposit:||This plasmid was deposited by Dr Kuan-Teh Jeang(1).
(1) NIH, Bethesda, Maryland, USA
|Related plasmid reference:||Sadowski et al., Nucleic Acids Res. 17 (1989), 7539 [PMID: 2798115]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pSV/TXBP15-1-AS (LMBP 3515) is available at BCCM/GeneCorner. This plasmid was deposited by Dr Kuan-Teh Jeang.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.