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GeneCorner plasmid details

Last data update: 18 January 2021 04:24 CET

Plasmid name: pSV23 (LMBP 1194)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: psv23.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pSV22S; pURSV(A)tss-D6
Further information: pSV23 is derived from pSV22S by deleting the small t-antigen splicing signal as a MboI fragment (corresponding to the MboI sites at nucleotide positions 4100 and 4710 of SV40).
Since prokaryotic part is derived from pAT153, there are no 'poison' sequences present.
Designed for transient expression in COS cells and constitutive expression in CHO cells (cotransfection with DHFR plasmids).
Expression sites: AccI, HindIII, SalI and XbaI which are all unique.
This plasmid does not contain the SV40 small t-antigen splicing signal.
It is presumably possible to clone into the unique AvrII and/or StuI site for expression, but this has not been tested.
Other name of the plasmid is pSV2-3tss-.
EMBL Accession number: -
Latest sequence update: 08/03/1989
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by D. Huylebroeck(1) and Prof. Dr W. Min Jou(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Danny Huylebroeck (1985)
Related plasmid reference: Huylebroeck et al., Gene 66 (1988), 163-181 [PMID: 2844629]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pSV23 (LMBP 1194) is available at BCCM/GeneCorner. This plasmid was deposited by D. Huylebroeck and Prof. Dr W. Min Jou and was published in Danny Huylebroeck, 1985.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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