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GeneCorner plasmid details

Last data update: 26 November 2020 04:30 CET

Plasmid name: pSV23E6Hf1 (LMBP 1979)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p1979.gb
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; truncated heavy chain of the Fab fragment E6F1 (E6Hf1)
Promoter: Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pSV23; pGEME6Hf1
Further information: The plasmid was constructed by inserting the HindIII-XbaI fragment of pGEME6Hf1, containing the cDNA copy of the truncated heavy chain E6Hf1 of the mouse Fab fragment E6F1, into the unique XbaI and HindIII expression sites of pSV23.
This plasmid is similar to pSV23SE6Hf1, but does not contain the SV40 small t-antigen splicing signal.
The translation stops at the beginning of the hinge region and therefore, no cysteine residues involved in the inter-heavy-chain disulfide bonds are present.
The plasmid is designed for transient expression in COS cells or constitutive expression in CHO cells (cotransfection with DHFR plasmids).
EMBL Accession number: -
Latest sequence update: 19/04/1990
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by P. Casneuf(1) and Prof. Dr W. Fiers(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: De Sutter et al., Gene 113 (1992), 223-230 [PMID: 1572543]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pSV23E6Hf1 (LMBP 1979) is available at BCCM/GeneCorner. This plasmid was deposited by P. Casneuf and Prof. Dr W. Fiers.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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