Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV23E6Hy3 (LMBP 1684)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p1684.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) half genomic gene; mouse::human chimeric heavy chain (E6Hy3) |
Promoter: | Simian virus 40 early promoter (SV40 early) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pSV23; pATE6Hy3 |
Further information: | This plasmid contains a half-genomic insert encoding the complete mouse::human chimeric heavy chain of the anti-hPLAP antibody E6(Hy3,κ) clockwise orientated relative to the SV40 early promoter. The mouse part of the cloned gene consists of cDNA; the human part consists of genomic DNA. The plasmid doesn't contain the SV40 small t-antigen splicing signal. Designed for transient expression in COS cells or constitutive expression in CHO cells (cotransfection with DHFR plasmids). The HindIII-SalI fragment of pATE6Hy3 is cloned in the HindIII-SalI site of pSV23. Other name of the plasmid is pSV23Hy3. |
EMBL Accession number: | - |
Latest sequence update: | 04/04/1989 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Related plasmid reference: | De Sutter et al., Gene 113 (1992), 223-230 [PMID: 1572543] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12xB HB101 |
Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV23E6Hy3 (LMBP 1684) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.