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GeneCorner plasmid details

Last data update: 23 September 2021 14:51 CEST

Plasmid name: pSV23S (LMBP 1195)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p1195.gb (View with Genome Compiler)
p1195.txt
p1195.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pSV22S; pURSV(A)tss+C4
Further information: Designed for transient expression in COS cells and constitutive expression in CHO cells (cotransfection with DHFR plasmids).
Expression sites: AccI, HindIII, SalI and XbaI which are all unique.
It is presumably possible to clone into the unique AvrII and/or unique StuI sites for expression, but this has not been tested.
The plasmid contains the SV40 small t-antigen splicing signal.
Since the prokaryotic part is derived from pAT153, there are no 'poison' sequences present.
Other name of the plasmid is pSVd2-3tss+.
EMBL Accession number: -
Latest sequence update: 16/02/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindII, PstI and SalI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by D. Huylebroeck(1) and Prof. Dr W. Min Jou(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Danny Huylebroeck (1985)
Related plasmid reference: Huylebroeck et al., Gene 66 (1988), 163-181 [PMID: 2844629]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 K514(λ)
Host reference: Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: Take a low-copy-plasmid yield into account while choosing a plasmid DNA isolation procedure.
Other culture collection numbers: -

Refer in your Materials and Methods:

pSV23S (LMBP 1195) is available at BCCM/GeneCorner. This plasmid was deposited by D. Huylebroeck and Prof. Dr W. Min Jou and was published in Danny Huylebroeck, 1985.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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