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GeneCorner plasmid details

Last data update: 23 September 2021 14:51 CEST

Plasmid name: pSV23SE6Hm (LMBP 2153)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2153.gb (View with Genome Compiler)
p2153.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; heavy chain (E6H), mutated sequence
Promoter: Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pSV23SE6H
Further information: This plasmid was derived from pSV23SE6H by introducing a unique KpnI site in front of the start of the mature E6H gene via site-specific mutagenesis.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727624.1.
Other names of the plasmid are pSV23SE6H2 and pSV23SE6Hm1.
EMBL Accession number: LT727624.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 02/04/1990
Sequence detail:
Nucleotide sequence after site-specific mutagenesis:

          pSV23SE6H                          pSV23SE6Hm

     Leader    |     VH                 Leader    |     VH
               |                                  |
5' GTC CAA TCC | CAG GTT CAG 3'    5' GTC CGG TAC | CAG GTT CAG 3'
   Val Gln Ser | Gln Val Gln               **  *  |               
                                      Val Arg Tyr | Gln Val Gln
                                           KpnI

*: Mutated nucleotide.
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI/HindIII, BglI/XmnI, ClaI/RsaI, EcoRI, KpnI/SacI, NcoI/XbaI, SalI and TaqI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr K. De Sutter(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB HB101
Host reference: Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022]
Related host reference: Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pSV23SE6Hm (LMBP 2153) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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