Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV23SE6Lm (LMBP 2152)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2152.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; light chain (E6L), mutated sequence |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23SE6L |
| Further information: | This plasmid was derived from pSV23SE6L by introducing a BsmI site in front of the start of the mature E6L gene via site-specific mutagenesis. Other name of the plasmid is pSV23SE6L2. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/04/1990 |
| Sequence detail: | Nucleotide sequence after site-specific mutagenesis:
pSV23SE6L pSV23SE6Lm
Leader | VL Leader | VL
| |
5' GCC AGA TGT | GAC ATC CAG 3' 5' GCG AAT GCT | GAC ATC CAG 3'
Ala Arg Cys | Asp Ile Gln * ** ** |
Ala Asn Ala | Asp Ile Gln
BsmI
*: Mutated nucleotide. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by W. Lammerant(1) (2) and Prof. Dr W. Fiers(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium. |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV23SE6Lm (LMBP 2152) is available at BCCM/GeneCorner. This plasmid was deposited by W. Lammerant and Prof. Dr W. Fiers . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.