GeneCorner plasmid details

Last data update: 21 October 2021 10:13 CEST

Plasmid name: pSV23SMTNF (LMBP 2171)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: (View with Genome Compiler)
analysis results


Cloned DNA: Mouse tumor necrosis factor cDNA (Tnf, TNF)
Promoter: Simian virus 40 early promoter (SV40 early)
binding site:
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pSV23S; pATMTNF3
Further information: This plasmid was constructed as follows: the NarI-EcoRI mouse TNF fragment from pATMTNF3 was blunt-ended (filled-in with Klenow DNA polymerase) and provided with SalI linkers (CGTCGACG); then, this fragment was inserted into the unique SalI site of pSV23S.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727628.1.
The 5' and the 3' borders of the insert were sequenced by LMBP.
The nucleotide sequence of the mouse TNF corresponds with the EMBL Nucleotide Sequence Database accession number M11731.1.
Name mentioned in Fransen et al. (1985) is pSVd2-3 mTNF.
Other names of the plasmid are pSV23SMuTNFwT and pSV2d-mTNF.
EMBL Accession number: M11731.1, view at EMBL, GenBank, DDBJ
LT727628.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 22/11/2007
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, SalI and StuI/XcmI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr E. Decoster(1) (2) and Prof. Dr W. Fiers(1) (2).
(1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: Fransen et al., Nucleic Acids Res. 13 (1985), 4417-4429 [PMID: 2989794]
Related plasmid reference: Seyhan et al., RNA 11 (2005), 837-846 [PMID: 15840823] [DOI: 10.1261/rna.7285805]
Enderlin et al., Cancer Gene Ther. 16 (2009), 49-60 [PMID: 18670452] [DOI: 10.1038/cgt.2008.62]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pSV23SMTNF (LMBP 2171) is available at BCCM/GeneCorner. This plasmid was deposited by Dr E. Decoster and Prof. Dr W. Fiers and was published in Fransen et al., 1985.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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