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GeneCorner plasmid details

Last data update: 18 January 2021 04:24 CET

Plasmid name: pSV25S (LMBP 1990)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: psv25s.gb (View with Genome Compiler)
psv25s.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Simian virus 40 early promoter (SV40 early)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pSV23S
Further information: pSV25S differs from pSV23S in having a unique E-KpnI site and missing the E-XbaI site.
pSV25S contains the SV40 small t-antigen splicing signal.
Expression sites: AccI, HindIII, KpnI and SalI which are all unique.
Designed for transient expression in COS cells and constitutive expression in CHO cells (cotransfection with DHFR plasmids).
Since the prokaryotic part is derived from pAT153, there are no 'poison' sequences present.
It is presumably possible to clone into the unique AvrII and/or unique StuI sites for expression, but this has not been tested.
Other name of the plasmid is pSV2-5tss+.
EMBL Accession number: -
Latest sequence update: 08/03/1989
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: HindIII and RsaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by D. Huylebroeck(1) and Prof. Dr W. Min Jou(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: PhD thesis Danny Huylebroeck (1985)
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pSV25S (LMBP 1990) is available at BCCM/GeneCorner. This plasmid was deposited by D. Huylebroeck and Prof. Dr W. Min Jou and was published in Danny Huylebroeck, 1985.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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