Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV529 (LMBP 1827)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
psv529.gb
(View with Genome Compiler) psv529.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Simian virus 40 early promoter (SV40 early) Simian virus 40 late promoter (SV40 late) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli; preferably recombination-deficient strains Mammalian cells; SV40 permissive cells |
| Parental clone: | pBR322; SV40; pSV5 |
| Further information: | SV40 DNA information starts at the SV40 HpaII site which was fused to the ClaI site of pBR322. This plasmid is a basic vector for gene expression under direction of the SV40 late promoter (requires permissive cells, i.e. AGMK derivatives). An intron is excised from the 5' UTR of the major SV40 late transcripts. The most important transcription initiation site is located at nucleotide position 4258 (cfr. L325 in SV40). The SV40 late transcripts have multiple cap sites. The first SV40 early region of this vector is intact (it codes for the large T-antigen), so that the plasmid can replicate in all SV40 permissive cells. Since 39% of the SV40 genome is duplicated (early region), one should be aware of the possibility of deletions due to homologous recombination. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727591.1. |
| EMBL Accession number: | LT727591.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/03/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BamHI/EcoRI, BanI and EcoRI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by D. Gheysen(1) and Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Gheysen et al., J. Mol. Appl. Genet. 1 (1982), 385-394 [PMID: 6296249] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV529 (LMBP 1827) is available at BCCM/GeneCorner. This plasmid was deposited by D. Gheysen and Prof. Dr W. Min Jou and was published in Gheysen et al., 1982. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.