Last data update: 24 January 2024 16:39 CET
Plasmid name: pTT3-DrGdf11-prey (LMBP 11689)
| New search | Print data sheet |
| Price category: | Cat. 2 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
Sanger: ef32240764.fasta Sanger: ef32240985.fasta |
| Cloned DNA: |
Danio rerio growth differentiation factor 11 cDNA (gdf11, GeneID 406247); extracellular domain (ECD) Rattus norvegicus Cd4 molecule cDNA (Cd4, GeneID 24932); fragment Rattus norvegicus cartilage oligomeric matrix protein cDNA (Comp, GeneID 25304); fragment |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Epstein-Barr virus origin of replication (oriP) |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | unc5bCD4d3+4-blac |
| Further information: | The plasmid was constructed by cloning the D. rerio Gdf11 extracellular domain coding sequence into the unc5bCD4d3+4-blac vector for Avidity-based Extracellular Interaction Screening (AVEXIS) assays. This vector expresses the D. rerio Gdf11 as a prey protein, linked to a tag containing domains 3 and 4 of the rat CD4 (Cd4d3+4) protein, followed by a peptide sequence from the rat cartilage oligomeric matrix protein (COMP) and a C-terminal β-lactamase enzyme. The COMP peptide ensures the prey forms pentamers once expressed. The unc5bCD4d3+4-blac vector was derived from pTT3, obtained from Dr Yves Durocher (National Research Council of Canada, Biotechnology Research Institute). The nucleotide sequence of the D. rerio Gdf11 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number KM655715.1. Other name of the plasmid is Gdf11-blac-AVEXIS. |
| EMBL Accession number: | KM655715.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 24/02/2020 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr U. Irion(1). It was constructed by C. Söllner(1). (1) Max Planck Institute for Developmental Biology, Tübingen, Germany |
| Plasmid reference: | Gao et al., Cell Rep. 12 (2015), 694-708 [PMID: 26190107] [DOI: 10.1016/j.celrep.2015.06.047] |
| Related plasmid reference: | Söllner et al., Genome Biol. 10 (2009), R99 [PMID: 19765300] [DOI: 10.1186/gb-2009-10-9-r99] Liu et al., Neuron 97 (2018), 1261-1267 [PMID: 29503192] [DOI: 10.1016/j.neuron.2018.02.010] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pTT3-DrGdf11-prey (LMBP 11689) is available at BCCM/GeneCorner. This plasmid was deposited by Dr U. Irion and was published in Gao et al., 2015. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.