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GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pTT3-DrMet-prey (LMBP 11679)

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Price category: Cat. 3 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

NGS: b12-gc-dec2019.fasta

Cloned DNA: Danio rerio MET proto-oncogene receptor tyrosine kinase cDNA (met, GeneID 100150664); extracellular domain (ECD)
Rattus norvegicus Cd4 molecule cDNA (Cd4, GeneID 24932); fragment
Rattus norvegicus cartilage oligomeric matrix protein cDNA (Comp, GeneID 25304); fragment
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Epstein-Barr virus origin of replication (oriP)
Host range: Escherichia coli
Mammalian cells
Parental clone: unc5bCD4d3+4-blac
Further information: The plasmid was constructed by cloning the D. rerio Met extracellular domain coding sequence into the unc5bCD4d3+4-blac vector for Avidity-based Extracellular Interaction Screening (AVEXIS) assays.
This vector expresses the D. rerio Met as a prey protein, linked to a tag containing domains 3 and 4 of the rat CD4 (Cd4d3+4) protein, followed by a peptide sequence from the rat cartilage oligomeric matrix protein (COMP) and a C-terminal β-lactamase enzyme. The COMP peptide ensures the prey forms pentamers once expressed.
The unc5bCD4d3+4-blac vector was derived from pTT3, obtained from Dr Yves Durocher (National Research Council of Canada, Biotechnology Research Institute).
The nucleotide sequence of the D. rerio Met cDNA corresponds with the EMBL Nucleotide Sequence Database accession number KM655614.1.
Other name of the plasmid is Met-blac-AVEXIS.
EMBL Accession number: KM655614.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 24/02/2020
Authenticity test: This plasmid has been fully sequenced but the NGS sequence data still need to be implemented.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr U. Irion(1). It was constructed by C. Söllner(1).
(1) Max Planck Institute for Developmental Biology, Tübingen, Germany
Plasmid reference: Gao et al., Cell Rep. 12 (2015), 694-708 [PMID: 26190107] [DOI: 10.1016/j.celrep.2015.06.047]
Related plasmid reference: Söllner et al., Genome Biol. 10 (2009), R99 [PMID: 19765300] [DOI: 10.1186/gb-2009-10-9-r99]
Liu et al., Neuron 97 (2018), 1261-1267 [PMID: 29503192] [DOI: 10.1016/j.neuron.2018.02.010]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5αT1R
Host reference: -
Related host reference: Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pTT3-DrMet-prey (LMBP 11679) is available at BCCM/GeneCorner. This plasmid was deposited by Dr U. Irion and was published in Gao et al., 2015.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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