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GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pTT3-DrSc:d0347-prey (LMBP 11946)

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Price category: Cat. 2 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

Sanger: ef32242440.fasta

Sanger: ef32242489.fasta

Cloned DNA: Danio rerio trophoblast glycoprotein b cDNA (tpbgb, sc:d0323, sc:d0347, GeneID 792712); extracellular domain (ECD)
Rattus norvegicus Cd4 molecule cDNA (Cd4, GeneID 24932); fragment
Rattus norvegicus cartilage oligomeric matrix protein cDNA (Comp, GeneID 25304); fragment
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Epstein-Barr virus origin of replication (oriP)
Host range: Escherichia coli
Mammalian cells
Parental clone: unc5bCD4d3+4-blac
Further information: The plasmid was constructed by cloning the D. rerio Tpbgb extracellular domain coding sequence into the unc5bCD4d3+4-blac vector for Avidity-based Extracellular Interaction Screening (AVEXIS) assays.
This vector expresses the D. rerio Tpbgb, as a prey protein, linked to a tag containing domains 3 and 4 of the rat CD4 (Cd4d3+4) protein, followed by a peptide sequence from the rat cartilage oligomeric matrix protein (Comp) and a C-terminal β-lactamase enzyme. The COMP peptide ensures the prey forms pentamers once expressed.
The unc5bCD4d3+4-blac vector was derived from pTT3, obtained from Dr Yves Durocher (National Research Council of Canada, Biotechnology Research Institute).
The nucleotide sequence of the D. rerio Tpbgb cDNA corresponds with the EMBL Nucleotide Sequence Database accession number CU468771.1.
Other name of the plasmid is sc:d0347-blac-AVEXIS.
EMBL Accession number: CU468771.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/04/2020
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr G. Wright(1). It was constructed by Dr C. Söllner(1).
(1) Cell Surface Signalling Laboratory, Wellcome Trust Sanger Institute, Cambridge, UK
Plasmid reference: Söllner et al., Genome Biol. 10 (2009), R99 [PMID: 19765300] [DOI: 10.1186/gb-2009-10-9-r99]
Related plasmid reference: Martin et al., Mol. Cell. Proteomics 9 (2010), 2654-2665 [PMID: 20802085] [DOI: 10.1074/mcp.M110.004119]
Bushell et al., Genome Res. 18 (2008), 622-630 [PMID: 18296487] [DOI: 10.1101/gr.7187808]
Gao et al., Cell Rep. 12 (2015), 694-708 [PMID: 26190107] [DOI: 10.1016/j.celrep.2015.06.047]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5αT1R
Host reference: -
Related host reference: Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pTT3-DrSc:d0347-prey (LMBP 11946) is available at BCCM/GeneCorner. This plasmid was deposited by Dr G. Wright and was published in Söllner et al., 2009.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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