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GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pTT3-hDCC-FN456-prey (LMBP 11526)

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Price category: Cat. 2 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p11526.gb
Sequence
analysis results
Genecorner:

Sanger: ef32243352.fasta

Sanger: ef32243373.fasta

Cloned DNA: Human DCC netrin 1 receptor cDNA (DCC, CRC18, CRCR1, MRMV1, HGPPS2, IGDCC1, NTN1R1, GeneID 1630); fragment
Rattus norvegicus Cd4 molecule cDNA (Cd4, GeneID 24932); fragment
Rattus norvegicus cartilage oligomeric matrix protein cDNA (Comp, GeneID 25304); fragment
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Epstein-Barr virus origin of replication (oriP)
Host range: Escherichia coli
Mammalian cells
Parental clone: unc5bCD4d3+4-blac
Further information: The plasmid was constructed by cloning part of the human DCC coding sequence into the unc5bCD4d3+4-blac vector for Avidity-based Extracellular Interaction Screening (AVEXIS) assays.
This vector expresses the unliganded membrane-proximal domains FN4-FN5-FN6 of human DCC as a prey protein, linked to a tag containing domains 3 and 4 of the rat Cd4 (Cd4d3+4) protein, followed by a peptide sequence from the rat cartilage oligomeric matrix protein (Comp) and a C-terminal β-lactamase enzyme. The Comp peptide ensures the prey forms pentamers once expressed.
The unc5bCD4d3+4-blac vector was derived from pTT3, obtained from Dr Yves Durocher (National Research Council of Canada, Biotechnology Research Institute).
Other name of the plasmid is DCC_FN456-blac-AVEXIS.
EMBL Accession number: -
Latest sequence update: 22/01/2020
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr X. Gao(1) and Dr R. Meijers(1).
(1) EMBL Hamburg, Germany
Plasmid reference: Finci et al., Protein Cell 8 (2017), 701-705 [PMID: 28664251] [DOI: 10.1007/s13238-017-0439-x]
Related plasmid reference: Söllner et al., Genome Biol. 10 (2009), R99 [PMID: 19765300] [DOI: 10.1186/gb-2009-10-9-r99]
Gao et al., Cell Rep. 12 (2015), 694-708 [PMID: 26190107] [DOI: 10.1016/j.celrep.2015.06.047]
Liu et al., Neuron 97 (2018), 1261-1267 [PMID: 29503192] [DOI: 10.1016/j.neuron.2018.02.010]
Restricted distribution: - BCCM/MTA adapted by EMBL
- Restricted to academic users
- Acknowledgment of the depositor in any publication resulting from the use of the MATERIAL
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5αT1R
Host reference: -
Related host reference: Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pTT3-hDCC-FN456-prey (LMBP 11526) is available at BCCM/GeneCorner. This plasmid was deposited by Dr X. Gao and Dr R. Meijers and was published in Finci et al., 2017.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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