GREAT AT SMALL THINGS

0

GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pTT3-hDRAXIN-bait (LMBP 11507)

Add to cart

New search Print data sheet
Price category: Cat. 2 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p11507.gb
Sequence
analysis results
Genecorner:

Sanger: ef32243335.fasta

Sanger: ef32243357.fasta

Cloned DNA: Human dorsal inhibitory axon guidance protein cDNA (DRAXIN, NEUCRIN, GeneID 374946)
Histidine tag (His-tag); C-terminal
Rattus norvegicus Cd4 molecule cDNA (Cd4, GeneID 24932); fragment
Biotinylation sequence (bio-tag); C-terminal
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: Rabbit β-globin polyadenylation signal (β-globin polyA)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Epstein-Barr virus origin of replication (oriP)
Host range: Escherichia coli
Mammalian cells
Parental clone: unc5bCD4d3+4-bio
Further information: The plasmid was constructed by cloning the human DRAXIN coding sequence into the unc5bCD4d3+4-bio vector for Avidity-based Extracellular Interaction Screening (AVEXIS) assays.
This vector expresses the human DRAXIN as a bait protein, linked to a tag containing domains 3 and 4 of the rat Cd4 (Cd4d3+4) protein, followed by a biotinylation sequence that is a substrate for the E.coli BirA enzyme and can therefore be monobiotinylated on a specific lysine residue. The Cd4d3+4 tag is recognised by the OX68 monoclonal antibody and is used to quantify the expression of the bait proteins by ELISA. The bait proteins are therefore monomeric and monobiotinylated.
The unc5bCD4d3+4-bio vector was derived from pTT3, obtained from Dr Yves Durocher (National Research Council of Canada, Biotechnology Research Institute).
Other name of the plasmid is DRAXIN-bio-AVEXIS.
EMBL Accession number: -
Latest sequence update: 09/09/2019
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr X. Gao(1) and Dr R. Meijers(1).
(1) EMBL Hamburg, Germany
Plasmid reference: Gao et al., Cell Rep. 12 (2015), 694-708 [PMID: 26190107] [DOI: 10.1016/j.celrep.2015.06.047]
Related plasmid reference: Söllner et al., Genome Biol. 10 (2009), R99 [PMID: 19765300] [DOI: 10.1186/gb-2009-10-9-r99]
Bushell et al., Genome Res. 18 (2008), 622-630 [PMID: 18296487] [DOI: 10.1101/gr.7187808]
Restricted distribution: - BCCM/MTA adapted by EMBL
- Restricted to academic users
- Acknowledgment of the depositor in any publication resulting from the use of the MATERIAL
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5αT1R
Host reference: -
Related host reference: Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pTT3-hDRAXIN-bait (LMBP 11507) is available at BCCM/GeneCorner. This plasmid was deposited by Dr X. Gao and Dr R. Meijers and was published in Gao et al., 2015.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

New search