Last data update: 24 January 2024 16:39 CET
Plasmid name: pTT3-hROBO4-prey (LMBP 11522)
New search | Print data sheet |
Price category: | Cat. 2 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p11522.gb |
Sequence analysis results Genecorner: |
Sanger: ef32243349.fasta Sanger: ef32243370.fasta |
Cloned DNA: |
Human pregnancy specific beta-1-glycoprotein 1 cDNA (PSG1, B1G1, PBG1, PSBG1, PS-beta-G-1, GeneID 5669); signal sequence Human roundabout guidance receptor 4 cDNA (ROBO4, MRB, AOVD3, ECSM4, GeneID 54538); extracellular domain (ECD) Rattus norvegicus Cd4 molecule cDNA (Cd4, GeneID 24932); fragment Rattus norvegicus cartilage oligomeric matrix protein cDNA (Comp, GeneID 25304); fragment |
Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli lac operon promoter |
Ribosome binding site: |
- |
Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Epstein-Barr virus origin of replication (oriP) |
Host range: | Escherichia coli Mammalian cells |
Parental clone: | unc5bCD4d3+4-blac |
Further information: | The plasmid was constructed by cloning the human ROBO4 extracellular domain coding sequence into the unc5bCD4d3+4-blac vector for Avidity-based Extracellular Interaction Screening (AVEXIS) assays. This vector expresses the ectodomain of the human ROBO4 as a prey protein, linked to a tag containing domains 3 and 4 of the rat Cd4 (Cd4d3+4) protein, followed by a peptide sequence from the rat cartilage oligomeric matrix protein (Comp) and a C-terminal β-lactamase enzyme. The Comp peptide ensures the prey forms pentamers once expressed. The unc5bCD4d3+4-blac vector was derived from pTT3, obtained from Dr Yves Durocher (National Research Council of Canada, Biotechnology Research Institute). Other name of the plasmid is ROBO4-blac-AVEXIS. |
EMBL Accession number: | - |
Latest sequence update: | 22/01/2020 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr X. Gao(1) and Dr R. Meijers(1). (1) EMBL Hamburg, Germany |
Plasmid reference: | - |
Related plasmid reference: | Söllner et al., Genome Biol. 10 (2009), R99 [PMID: 19765300] [DOI: 10.1186/gb-2009-10-9-r99] Gao et al., Cell Rep. 12 (2015), 694-708 [PMID: 26190107] [DOI: 10.1016/j.celrep.2015.06.047] Liu et al., Neuron 97 (2018), 1261-1267 [PMID: 29503192] [DOI: 10.1016/j.neuron.2018.02.010] |
Restricted distribution: | - BCCM/MTA adapted by EMBL - Restricted to academic users - Acknowledgment of the depositor in any publication resulting from the use of the MATERIAL |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5αT1R |
Host reference: | - |
Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pTT3-hROBO4-prey (LMBP 11522) is available at BCCM/GeneCorner. This plasmid was deposited by Dr X. Gao and Dr R. Meijers. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.