Last data update: 24 January 2024 16:39 CET
Plasmid name: pUC18 (LMBP 7874)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p7874.gb
(View with Genome Compiler) p7874.txt p7874.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli lac Z gene (lacZ); fragment, incl. α part |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains expressing the lacZ ω fragment (lacZΔM15) for screening |
| Parental clone: | pBR322 |
| Further information: | This plasmid was designed as an insert detection vector using α-complementation. pUC18 is suitable for the expression of fused proteins using the lac promoter and the lacZα part. pUC18 has the same polylinker as pUC19, but inverted. pUC18 has no PstI site in the ampicillin resistance gene. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number L08752.1, starting at another nucleotide position and complemented. |
| EMBL Accession number: | L08752.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 01/06/1990 |
| Sequence detail: | Nucleotide sequence around the polylinker:
5' ATG.ACC.ATG.ATT.ACG.AAT.TCG.AGC.TCG.GTA.CCC.GGG.GAT.CCT.CTA.
*** EcoRI SacI KpnI SmaI BamHI XbaI
GAG.TCG.ACC.TGC.AGG.CAT.GCA.AGC.TTG.GCA.CTG.GCC. 3'
HindII PstI SphI HindIII
SalI
***: start codon of lacZ alpha.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HincIII and HindII/XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Messing(1). (1) State University of New Jersey, Piscataway, USA |
| Plasmid reference: | Norrander et al., Gene 26 (1983), 101-106 [PMID: 6323249] |
| Related plasmid reference: | Debode et al., Anal. Bioanal. Chem. 396 (2010), 2151-2164 [PMID: 20099062] [DOI: 10.1007/s00216-009-3396-2] Broeders et al. |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | DSM 3424 NCCB 3030 PC-V 3030 LMBP 03662 LMBP 04483 |
Refer in your Materials and Methods: |
pUC18 (LMBP 7874) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Messing and was published in Norrander et al., 1983. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.