Last data update: 24 January 2024 16:39 CET
Plasmid name: pUC18gMIL6 (LMBP 2806)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2806.gb
(View with Genome Compiler) p2806.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse interleukin 6 genomic DNA (Il6, Il-6, GeneID 16193); fragment |
| Promoter: | Mouse interleukin 6 promoter (IL6) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC18; M13mp18gMIL6 |
| Further information: | pUC18gMIL6 was constructed as follows: the BamHI fragment of M13mp18gMIL6, containing the 5' flanking region, the promoter, the first and second exon, the entire first intron and part of the second intron of the genomic mouse interleukin 6 DNA (mIL6), was cloned anticlockwise into the unique BamHI site of pUC18. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727547.1. The nucleotide sequence of the genomic mouse interleukin 6 DNA corresponds with the Genbank accession number M20572.1. Other name of the plasmid is pUC18MIL6g. |
| EMBL Accession number: | M20572.1, view at EMBL, GenBank, DDBJ LT727547.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 13/10/2003 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, HindII, HindIII, NheI, NheI/XbaI, PstI, RsaI and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Tanabe et al., J. Immunol. 141 (1988), 3875-3881 [PMID: 3263439] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUC18gMIL6 (LMBP 2806) is available at BCCM/GeneCorner. This plasmid was deposited by Dr W. Vanden Berghe and Prof. Dr G. Haegeman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.