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GeneCorner plasmid details

Last data update: 09 March 2021 04:23 CET

Plasmid name: pUC18gMIL6 (LMBP 2806)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p2806.gb (View with Genome Compiler)
p2806.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Mouse interleukin 6 genomic DNA (Il6, Il-6, GeneID 16193); fragment
Promoter: Mouse interleukin 6 promoter (IL6)
Escherichia coli lac operon promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pUC18; M13mp18gMIL6
Further information: pUC18gMIL6 was constructed as follows: the BamHI fragment of M13mp18gMIL6, containing the 5' flanking region, the promoter, the first and second exon, the entire first intron and part of the second intron of the genomic mouse interleukin 6 DNA (mIL6), was cloned anticlockwise into the unique BamHI site of pUC18.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727547.1.
The nucleotide sequence of the genomic mouse interleukin 6 DNA corresponds with the Genbank accession number M20572.1.
Other name of the plasmid is pUC18MIL6g.
EMBL Accession number: M20572.1, view at EMBL, GenBank, DDBJ
LT727547.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 13/10/2003
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, HindII, HindIII, NheI, NheI/XbaI, PstI, RsaI and XbaI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr W. Vanden Berghe(1) and Prof. Dr G. Haegeman(1).
(1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Related plasmid reference: Tanabe et al., J. Immunol. 141 (1988), 3875-3881 [PMID: 3263439]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pUC18gMIL6 (LMBP 2806) is available at BCCM/GeneCorner. This plasmid was deposited by Dr W. Vanden Berghe and Prof. Dr G. Haegeman.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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