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GeneCorner plasmid details

Last data update: 17 September 2021 10:55 CEST

Plasmid name: pUC19 (LMBP 5026)

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Price category: Cat. 3 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p5026.gb (View with Genome Compiler)
p5026.txt
p5026.pdf
Sequence
analysis results
Genecorner:

NGS: 5026-gc-jun2020.fasta

Cloned DNA: Escherichia coli lac Z gene (lacZ); fragment, incl. α part
Promoter: Escherichia coli lac operon promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains expressing the lacZ ω fragment (lacZΔM15) for screening
Parental clone: pBR322
Further information: This plasmid was designed as an insert detection vector using α-complementation.
pUC19 is suitable for the expression of fused proteins using the lac promoter and the lacZα part.
pUC19 has the same polylinker as pUC18, but inverted.
pUC19 has no PstI site in the ampicillin resistance gene.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number M77789.1, starting at another nucleotide position and complemented.
EMBL Accession number: M77789.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 15/12/2020
Sequence detail:
Nucleotide sequence around the polylinker:

5' ATG.ACC.ATG.ATT.ACG.CCA.AGC.TTG.CAT.GCC.TGC.AGG.TCG.ACT.CTA.
   ***                   HindIII SphI    PstI    SalI    XbaI
                                                 HindII
                                                           
   GAG.GAT.CCC.CGG.GTA.CCG.AGC.TCG.AAT.TCA.CTG.GCC. 3'
     BamHI  SmaI KpnI    SacI    EcoRI
                                       
*: start codon of the lacZ gene.
Punctuation indicates reading frame.
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, BglI, HindII/XmnI, PstI, PvuII and RsaI.

This plasmid has also been fully sequenced.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Messing(1).
(1) Waksman Institute, Rutgers, State University of New Jersey, Piscataway, USA
Plasmid reference: Norrander et al., Gene 26 (1983), 101-106 [PMID: 6323249]
Related plasmid reference: Yanisch-Perron et al., Gene 33 (1985), 103-119 [PMID: 2985470]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: DSM 3425
NCCB 3031
PC-V 3031
LMBP 01128

Refer in your Materials and Methods:

pUC19 (LMBP 5026) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Messing and was published in Norrander et al., 1983.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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