Last data update: 18 January 2021 04:24 CET
Plasmid name: pUC19 (LMBP 5026)
|Price category:||Cat. 3 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
Escherichia coli lac Z gene (lacZ); fragment, incl. α part|
|Promoter:||Escherichia coli lac operon promoter|
|Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)|
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli; use strains expressing the lacZ ω fragment (lacZΔM15) for screening|
|Further information:||This plasmid was designed as an insert detection vector using α-complementation.
pUC19 is suitable for the expression of fused proteins using the lac promoter and the lacZα part.
pUC19 has the same polylinker as pUC18, but inverted.
pUC19 has no PstI site in the ampicillin resistance gene.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number M77789.1, starting at another nucleotide position and complemented.
|EMBL Accession number:||M77789.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||15/12/2020|
Nucleotide sequence around the polylinker: 5' ATG.ACC.ATG.ATT.ACG.CCA.AGC.TTG.CAT.GCC.TGC.AGG.TCG.ACT.CTA. *** HindIII SphI PstI SalI XbaI HindII GAG.GAT.CCC.CGG.GTA.CCG.AGC.TCG.AAT.TCA.CTG.GCC. 3' BamHI SmaI KpnI SacI EcoRI *: start codon of the lacZ gene. Punctuation indicates reading frame.
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BamHI, BglI, HindII/XmnI, PstI, PvuII and RsaI.
This plasmid has also been fully sequenced.
|History of deposit:||This plasmid was deposited by Dr J. Messing(1).
(1) Waksman Institute, Rutgers, State University of New Jersey, Piscataway, USA
|Plasmid reference:||Norrander et al., Gene 26 (1983), 101-106 [PMID: 6323249]
|Related plasmid reference:||Yanisch-Perron et al., Gene 33 (1985), 103-119 [PMID: 2985470]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12|
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||DSM 3425
Refer in your Materials and Methods:
|pUC19 (LMBP 5026) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Messing and was published in Norrander et al., 1983.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.