Last data update: 25 October 2020 04:11 CET
Plasmid name: pbin8LefdGFP (LMBP 6843)
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6843.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: | Aequorea victoria green fluorescent protein DNA (GFP); destabilized enhanced sequence (d2EGFP) |
| Promoter: | Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) Phage SP6 promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pbin8 |
| Further information: | - |
| EMBL Accession number: | - |
| Latest sequence update: | 01/03/2011 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr K. Vleminckx(1) (2). It was constructed by Dr H.T. Tran(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Tran et al., Proc. Natl. Acad. Sci. U.S.A. 107 (2010), 16160-16165 [PMID: 20805504] [DOI: 10.1073/pnas.1007725107] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
|
Refer in your Materials and Methods: |
pbin8LefdGFP (LMBP 6843) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. Vleminckx and was published in Tran et al., 2010. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.