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GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pPTK081_0_Empty (LMBP 12734)

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Price category: Cat. OP (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p12734.gb (View with Genome Compiler)
p12734.txt
p12734.pdf
Manual Download OPENPichia tutorial
Sequence
analysis results
Genecorner:

Sanger: ef32243389.fasta

Sanger: ef32243456.fasta

NGS: 12734-ps-gc-sep2023.fasta

Cloned DNA: Synthetic green fluorescent protein DNA (GFP); superfolder GFP (sfGFP)
Promoter: Escherichia coli glycerol-3-phosphate transporter promoter (glpT)
Ribosome
binding site:
Ribosome binding site (RBS); synthetic sequence
Terminator: Escherichia coli rrnB operon T1 terminator
Phage T7 early transcription terminator (T7Te)
Selection marker: Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Pichia pastoris; as part of the OPENPichia plasmid kit
Parental clone: pPTK_0
Further information: The plasmid was constructed by cloning the synthetic sfGFP coding sequence, codon optimised for P. pastoris, in the synthetic pPTK_0 vector.
This plasmid is a level 0 destination vector for OPENPichia. It is intended to subclone new parts for this modular cloning technology.
Other name of the plasmid is pPTK081.
EMBL Accession number: -
Latest sequence update: 23/04/2021
Authenticity test: The glpT-RBS-sfGFP-T1-T7Te cassette has been sequenced at BCCM/GeneCorner.

The plasmid has also been fully sequenced.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by S. Vanmarcke(1)(2) and Prof. Dr N. Callewaert(1)(2). It was constructed by Dr K. Vandewalle(1)(2) and Dr R. Vanluchene(1)(2).
(1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium
(2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
Plasmid reference: Claes et al., Nature (2024) [PMID: 38443579] [DOI: 10.1038/s41564-023-01574-w]
Restricted distribution: - BCCM MTA adapted for OPENPichia
- RECIPIENT agrees to refer to the 'Plasmid reference' in the first publication making use of the MATERIAL.
- When appropriate in accordance with academic customs, RECIPIENT agrees to include the depositor(s) as co-author(s) in the first publication making use of the MATERIAL.
- When making use of the MATERIAL, RECIPIENT agrees to refer to ‘OPENPichia’ in the Materials and Methods section and in the acknowledgments of all publications, as well as when launching commercial products.
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5αT1R
Host reference: -
Related host reference: Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996]
Cultivation medium: LB-Lennox + chloramphenicol (34 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pPTK081_0_Empty (LMBP 12734) is available at BCCM/GeneCorner. This plasmid was deposited by S. Vanmarcke and Prof. Dr N. Callewaert and was published in Claes et al., 2024.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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