Last data update: 24 January 2024 16:39 CET
Plasmid name: pPTK081_0_Empty (LMBP 12734)
New search | Print data sheet |
Price category: | Cat. OP (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p12734.gb
(View with Genome Compiler) p12734.txt p12734.pdf |
Manual | Download OPENPichia tutorial |
Sequence analysis results Genecorner: |
Sanger: ef32243389.fasta Sanger: ef32243456.fasta |
Cloned DNA: |
Synthetic green fluorescent protein DNA (GFP); superfolder GFP (sfGFP) |
Promoter: | Escherichia coli glycerol-3-phosphate transporter promoter (glpT) |
Ribosome binding site: |
Ribosome binding site (RBS); synthetic sequence |
Terminator: | Escherichia coli rrnB operon T1 terminator Phage T7 early transcription terminator (T7Te) |
Selection marker: | Chloramphenicol (cam) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli Pichia pastoris; as part of the OPENPichia plasmid kit |
Parental clone: | pPTK_0 |
Further information: | The plasmid was constructed by cloning the synthetic sfGFP coding sequence, codon optimised for P. pastoris, in the synthetic pPTK_0 vector. This plasmid is a level 0 destination vector for OPENPichia. It is intended to subclone new parts for this modular cloning technology. Other name of the plasmid is pPTK081. |
EMBL Accession number: | - |
Latest sequence update: | 23/04/2021 |
Authenticity test: | The glpT-RBS-sfGFP-T1-T7Te cassette has been sequenced at BCCM/GeneCorner. The plasmid has also been fully sequenced. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by S. Vanmarcke(1)(2) and Prof. Dr N. Callewaert(1)(2). It was constructed by Dr K. Vandewalle(1)(2) and Dr R. Vanluchene(1)(2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
Plasmid reference: | Claes et al., Nature (2024) [PMID: 38443579] [DOI: 10.1038/s41564-023-01574-w] |
Restricted distribution: | - BCCM MTA adapted for OPENPichia - RECIPIENT agrees to refer to the 'Plasmid reference' in the first publication making use of the MATERIAL. - When appropriate in accordance with academic customs, RECIPIENT agrees to include the depositor(s) as co-author(s) in the first publication making use of the MATERIAL. - When making use of the MATERIAL, RECIPIENT agrees to refer to ‘OPENPichia’ in the Materials and Methods section and in the acknowledgments of all publications, as well as when launching commercial products. - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department. |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5αT1R |
Host reference: | - |
Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPTK081_0_Empty (LMBP 12734) is available at BCCM/GeneCorner. This plasmid was deposited by S. Vanmarcke and Prof. Dr N. Callewaert and was published in Claes et al., 2024. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.