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GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pPTK213_P3a_ScMFnoEAEA (LMBP 12747)

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Price category: Cat. 3 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: p12747.gb (View with Genome Compiler)
p12747.txt
p12747.pdf
Sequence
analysis results
Genecorner:

Sanger: ef32243405.fasta

Sanger: ef32243470.fasta

NGS: 12747-ps-gc-sep2023.fasta

Cloned DNA: Saccharomyces cerevisiae α-mating factor 1 gene (MFα1, GeneID 855914); prepro secretion signal sequence (ppMF), codon optimised
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Pichia pastoris; as part of the OPENPichia plasmid kit
Parental clone: pPTK_0
Further information: The plasmid was constructed by cloning the synthetic codon optimised S. cerevisiae ppMF signal sequence, without the 4 downstream codons encoding EAEA, in the synthetic pPTK_0 vector.
This plasmid contains a yeast signal sequence (OPENPichia part 3a) for the OPENPichia modular cloning technology.
OPENPichia parts 1 to 8 can be combined via BsaI restriction and ligation of the parts into a Pichia expression plasmid.
Alternatively, a transcriptional unit, consisting of OPENPichia parts 1 to 5 can be assembled via BsaI restriction and ligation. Several transcriptional units can be combined in a multigene destination vector via BsmBI restriction and ligation.
Other name of the plasmid is pPTK213.
EMBL Accession number: -
Latest sequence update: 06/05/2021
Authenticity test: The plasmid was sequenced at BCCM/GeneCorner, except for a small fragment upstream of the chloramphenicol resistance gene and the pMB1 ori.

The plasmid has also been fully sequenced.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by S. Vanmarcke(1)(2) and Prof. Dr N. Callewaert(1)(2). It was constructed by Dr K. Vandewalle(1)(2) and Dr R. Vanluchene(1)(2).
(1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium
(2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
Plasmid reference: Claes et al., Nature (2024) [PMID: 38443579] [DOI: 10.1038/s41564-023-01574-w]
Restricted distribution: - BCCM MTA adapted for OPENPichia
- RECIPIENT agrees to refer to the 'Plasmid reference' in the first publication making use of the MATERIAL.
- When appropriate in accordance with academic customs, RECIPIENT agrees to include the depositor(s) as co-author(s) in the first publication making use of the MATERIAL.
- When making use of the MATERIAL, RECIPIENT agrees to refer to ‘OPENPichia’ in the Materials and Methods section and in the acknowledgments of all publications, as well as when launching commercial products.
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5αT1R
Host reference: -
Related host reference: Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996]
Cultivation medium: LB-Lennox + chloramphenicol (34 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pPTK213_P3a_ScMFnoEAEA (LMBP 12747) is available at BCCM/GeneCorner. This plasmid was deposited by S. Vanmarcke and Prof. Dr N. Callewaert and was published in Claes et al., 2024.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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