Last data update: 24 January 2024 16:39 CET
Plasmid name: pPTK047_P4_AOX1tt (LMBP 12750)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p12750.gb
(View with Genome Compiler) p12750.txt p12750.pdf |
| Sequence analysis results Genecorner: |
Sanger: ef32243408.fasta Sanger: ef32243473.fasta Sanger: ef32708307.fasta Sanger: ef32708289.fasta Sanger: ef32708298.fasta Sanger: ef32708280.fasta |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) |
| Selection marker: | Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Pichia pastoris; as part of the OPENPichia plasmid kit |
| Parental clone: | pPTK_0 |
| Further information: | The plasmid was constructed by cloning the synthetic P. pastoris AOX1 terminator into the synthetic pPTK_0 vector. This plasmid contains a yeast terminator (OPENPichia part 4) for the OPENPichia modular cloning technology. OPENPichia part 4 connects directly to the gene of interest (OPENPichia part 3b or a custom part 3). To include a C-terminal marker (OPENPichia part 4a), choose a terminator from OPENPichia part 4b. OPENPichia parts 1 to 8 can be combined via BsaI restriction and ligation of the parts into a Pichia expression plasmid. Alternatively, a transcriptional unit, consisting of OPENPichia parts 1 to 5 can be assembled via BsaI restriction and ligation. Several transcriptional units can be combined in a multigene destination vector via BsmBI restriction and ligation. Other name of the plasmid is pPTK047. |
| EMBL Accession number: | - |
| Latest sequence update: | 06/05/2021 |
| Authenticity test: | This plasmid has been fully sequenced by BCCM/GeneCorner. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by S. Vanmarcke(1)(2) and Prof. Dr N. Callewaert(1)(2). It was constructed by Dr K. Vandewalle(1)(2) and Dr R. Vanluchene(1)(2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Claes et al., Nature (2024) [PMID: 38443579] [DOI: 10.1038/s41564-023-01574-w] |
| Restricted distribution: | - BCCM MTA adapted for OPENPichia - RECIPIENT agrees to refer to the 'Plasmid reference' in the first publication making use of the MATERIAL. - When appropriate in accordance with academic customs, RECIPIENT agrees to include the depositor(s) as co-author(s) in the first publication making use of the MATERIAL. - When making use of the MATERIAL, RECIPIENT agrees to refer to ‘OPENPichia’ in the Materials and Methods section and in the acknowledgments of all publications, as well as when launching commercial products. - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPTK047_P4_AOX1tt (LMBP 12750) is available at BCCM/GeneCorner. This plasmid was deposited by S. Vanmarcke and Prof. Dr N. Callewaert and was published in Claes et al., 2024. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.