LMG media details

Last data update: 20 October 2021 09:31 CEST  

1 medium found

Name: DNMS medium pH 5,8

Medium number: 1012

Recipe: dNMS Salt Stock:

MgSO4 x 7H2O 10,0 g

KNO3 10,0 g

CaCl2 x 2H2O 1,5 g

Distilled water 1 L

FeNaEDTA Stock

FeNaEDTA 0,5 g

Distilled water 100 ml

Na2HPO4 Stock

Na2HPO4 x 12H2O 71,7 g

Distilled water 1 L

KH2PO4 Stock

KH2PO4 27,2 g

Distilled water 1 L

1000x trace solution:

Na2EDTA x 2H2O 0,5 g

FeSO4 x 7H2O 0,2 g

H3BO3 0,03 g

CoCl2 x 6H2O 0,02 g

ZnSO4 x 7H2O 0,01 g

MnCl2 x 4H2O 0,003 g

Na2MoO4 x 2H2O 0,003 g

NiCl2 x 6H2O 0,002 g

CuSO4 x 5H2O 2,5 g

Distilled water 1 L

Dilute 20ml of DNMS salts stock to 800ml.

Add 1 ml FeNaEDTA Stock and 1 ml trace solution.

Dissolve and bring to 1 liter (distilled water) For solid media add 15g/l Bacto Agar.

After sterilization, cool to 50°C-60°C and add first 5ml KH2PO4 Stock (filter sterilized) followed by 5ml Na2HPO4 Stock (filter sterilized).

pH of the medium should be 5,8