Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | Medium for marine Planctomycetes |
| Medium number: | 1019 |
| Recipe: | Mineral salt sol. (Hutner/Basal Salt) 20 ml Peptone (Bacto) 1 g Yeast extract (Bacto) 1 g HEPES 2,38 g Artificial seawater 250 ml Distilled water up to 1L Adjust pH to 7.5 with 5 M KOH For plates: 15 g/l agar (Bacto) 3x washed with distilled water, autoclave separately. Sterilize 20 min./121°C. After cooling to 60°C add to the medium: Glucose sol. (25%, sterile-filtered) 4 ml Vitamin sol. 5 ml N-Acetylglucosamine 20 ml of 50 g/lstock sol. Trace element sol.1 ml Artificial seawater /l NaCl 46.94 g Na2SO4 7.84 g MgCl2 x 6H20 21.28 g CaCl2 x 2H20 2.86 g NaHCO3 0.384 g KCl 1.384 g KBr 0.192 g H3BO3 0.052 g SrCl2 x 6H20 0.08 g NaF 0.006 g Mineral salt sol. (Hutner/Basal Salt): NTA 10 g MgSO4 x 7H2O 29,70 g CaCl2 x 2H2O 3,34 g Na2MoO4 x 2H2O 12,67 mg FeSO4 x 7H2O 99 mg Metall salt sol. "44" 50 ml Distilled water add to 1L Dissolve NTA in 700 ml distill water by adjusting pH to 7.2 with KOH. Dissolve all components separately and combine slowly. Sterilize by filtration. Store at 4°C "Metals 44": Na-EDTA 250 mg ZnSO4 x 7H2O 1095 mg FeSO4 x 7H2O 500 mg MnSO4 x H2O 154 mg CuSO4 x 5H2O 39,5 mg CoCl2 x 6H2O 20,3 mg Na2B4O7 x 10H2O 17,7 mg Distilled water 1L Dissolve the EDTA in 700 ml and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metals if necessary. Store at 4°C. Do not autoclave. Vitamin solution: Biotin 4 mg Folic acid 4 mg Pyridoxine-HCl 20 mg Riboflavine 10 mg Thiamine-HCl 10 mg Nicotinamide 10 mg Ca-pantothenate 10 mg Vitamin B12 0,2 mg p-Aminobenzoic acid 10 mg Distilled water 1 L Dissolve p-Aminobenzoic acid first. Store in the dark and cold Trace element solution per liter: Na-Nitrilotriacetat 1500 mg MnSO4 x H2O 500 mg FeSO4 x7H2O 100 mg Co(NO3)2 x 6H20 100 mg ZnCl 100 mg NiCl2 x 6H2O 50 mg H2SeO3 50 mg CuSO4 x 5H2O 10 mg ALK(SO4)2 x12H2O 10 mg H3BO3 10 mg NaMo04 x 2H2O 10 mg Na2WO4x 2H2O 10 mg Sterilize by filtration. Store in the dark and cold |