Last data update:
24 January 2024 16:39 CET
1 medium found
Name: | Thermus medium pH 7,5 |
Medium number: | 1023 |
Recipe: | Yeast extract 1 g Triptone 1 g Thermus macronutrients solution 10x 100 ml Thermus micronutrients solution 100x 10 ml FeCl3SOlution 100x 10 ml Agar 15 g Distilled water up to 1 L Adjust pH to 7,5 with NaOH. Autoclave at 121°C for 15 minutes. Thermus macronutrients solution 10x: Nitrilotriacetic acid 1 g CaSO4 x 2H2O 0,6 g MgSO4 x 7H2O 1 g NaCl 0,08 g KNO3 1,03 g NaNO3 6,89 g Na2HPO4 1,11 g Distilled water 1 L Thermus micronutrients solution 100x: MnSO4 x H2O 220 mg ZnSO4 x 7H2O 50 mg H3BO3 50 mg CuSO4 x 5H2O 2,5 mg Na2MoO4 x 2H2O 2,5 mg CoCl2 x 6H2O 4,6 mg Distilled water 1 L FeCl3SOlution 100x: FeCl3 x 6H2O 46,6 mg Distilled water 1 L |