Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | GYS medium |
| Medium number: | 1027 |
| Recipe: | Solution A 500 ml Solution B 500 ml For solid medium add 2% agar. Solutions A and B are autoclaved separetely and combined before solidification (50 - 60°C) Solution A: KH2PO4 1 g (NH4)2SO4 1 g Yeast extract 0,3 g MgCl2 x 6H20 0,2 g CaCl2 45 mg NaCl 0,2 g Trace element 1 ml Distilled water 0,5 L Adjust pH to 4.5 with 1 N H2SO4 Solution B: Glucose 2 g Agar (if needed) 20 g Distilled water 0,5 L Trace Element solution: 2Na-EDTA 5 g FeCl2 x 4H20 1 g H3BO3 0,3 g CoCl2 x 6H2O 0,11 g ZnCl2 50 mg MnCl2 x 4H2O 30 mg Na2MoO4 x 2H2O 30 mg NiSO4 x 6H2O 30 mg CuCl2 x 2H2O 10 mg Distilled water 1 L |