GREAT AT SMALL THINGS

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LMG media details

Last data update: 18 June 2021 07:57 CEST  

1 medium found

Name: GYS medium

Medium number: 1027

Recipe: Solution A 500 ml

Solution B 500 ml



For solid medium add 2% agar. Solutions A and B are autoclaved separetely and combined before solidification (50 - 60°C)



Solution A:

KH2PO4 1 g

(NH4)2SO4 1 g

Yeast extract 0,3 g

MgCl2 x 6H20 0,2 g

CaCl2 45 mg

NaCl 0,2 g

Trace element 1 ml

Distilled water 0,5 L

Adjust pH to 4.5 with 1 N H2SO4



Solution B:

Glucose 2 g

Agar (if needed) 20 g

Distilled water 0,5 L



Trace Element solution:

2Na-EDTA 5 g

FeCl2 x 4H20 1 g

H3BO3 0,3 g

CoCl2 x 6H2O 0,11 g

ZnCl2 50 mg

MnCl2 x 4H2O 30 mg

Na2MoO4 x 2H2O 30 mg

NiSO4 x 6H2O 30 mg

CuCl2 x 2H2O 10 mg

Distilled water 1 L