GREAT AT SMALL THINGS

0

LMG media details

Last data update: 24 January 2024 16:39 CET  

1 medium found

Name: YTG medium

Medium number: 1029

Recipe: Yeast extract 1 g

Tryptone 1 g

Glucose 2.5 g

Sea salts 30 g

PIPES 6.05 g

Trace element solution 1 ml

Vitamin solution 10 ml

Cystein 0.2 g

Reazurin 1 mg

Distilled water 1 L

pH 6.8



Trace element solution (g/l)

(NH4)2Fe(S04)2 x 6H2O 0.78428

Na2SO4 0.28408

CoCl2 x 6H2O 0.23793

NiCl2 x 6H2O 0.23769

MnCl2 x 4H2O 0.098955

ZnSO4 x 7H2O 0.14378

Na2SeO3 0.09447

Na2MoO4 x 2H2O 0.024195

Na2WO4 x 2H2O 0.029386

H3BO3 0.006183

CuCl2 x 2H2O 0.0017048

The pH was adjusted to 7.0 at 25°C with 10% (wt/vol) NaOH



Vitamin solution (mg/l)

Biotin 2

Folic acid 2

Pyridoxine HCl 10

Riboflavin 5

Thiamin 5

Nicotinic acid 5

Pantothenic acid 5

Vitamin B12 0.1

P-aminobenzoic acid 5

Thioctic acid 5

Sterilize by filtration