Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | Modified NaCl-dANMS medium |
| Medium number: | 1035 |
| Recipe: | Stock solution 1 (50x salt stock) MgSO4 x7 H2O :10g/L CaCl2 x2 H2O : 1.5g/L Stock solution 2 :100mM FeNaEDTA Stock solution 3 : 100mM KH2PO4 Stock solution 4 : 10mM CuSO4 x 5H2O Trace elements solution (according to dNMS medium) Na2EDTA x2 H2O 0.5g/L FeSO4 x 7H2O 0.2g/L H3BO3 0.03g/L CoCL2 x 6H2O 0.02g/L ZnSO4 x 7H2O 0.01g/L MnCl2 x 4H2O 0.003g/L NaMoO4 x 2H2O 0.003g/L NiCl2 x 6H2O 0.002 g/L Lanthanide solution 100µM of the lanthanides CeCl3 x 7H2O, LaCl3, NdCl3 x 6H2O, PrCl3 Stock solution 5 : NaCl : 30g/100mL (30%) Stock solution 6 : 100mM NH4Cl Stock solution 7 : 100mM KNO3 Hepes solution (100mM) Medium preparation: dilute 20 ml of stock solution 1, 100 ml of stock solution 5, 50 ml of HEPES solution, 20 ml of solution 6 and 7 and 10 ml of solution 3 to 800 ml Add 1ml trace element solution Add 0,4 ml stock solution 2 Add 0,1 ml solution 4 add 1 ml Lanthanide solution Dissolve and bring to 1L Adjust pH to 7.8 with HCl or NaOH. Autoclave medium |