LMG media details

Last data update: 16 April 2021 10:30 CEST  

1 medium found

Name: Medium for Tundrispaera lichenicola

Medium number: 1037

Recipe: 0.1 g KH2PO4

0.1g (NH4)2SO4

10 ml Mineral salt sol. (Hutner/Basal Salt)

0.5 g N-acetylglucosamine

0.25 g glucose

0.2 g yeast extract

Vitamin solution N°6 (Staley, 1968)

pH 5.8-6.2

Vitamin solution N°6 (Staley, 1968)

Biotin 4 mg

Pyridoxine hydrochloride 20 mg

Thiamin hydrochloride 10 mg

Ca pantothenate 10 mg

p-Aminobenzoic acid 10 mg

Folic acid 4 mg

Riboflavin 10 mg

Nicotinic acid or nicotinamide 10 mg

Vitamin B12 0,2 mg

Distilled water 1 L

Sterilze by filtration only.

Mineral salt sol. (Hutner/Basal Salt):

NTA 10 g

MgSO4 x 7H2O 29,70 g

CaCl2 x 2H2O 3,34 g

Na2MoO4 x 2H2O 12,67 mg

FeSO4 x 7H2O 99 mg

Metall salt sol. "44" 50 ml

Distilled water add to 1L

Dissolve NTA in 700 ml distill water by adjusting pH to 7.2 with KOH. Dissolve all components separately and combine slowly. Sterilize by filtration. Store at 4°C

"Metals 44":

Na-EDTA 250 mg

ZnSO4 x 7H2O 1095 mg

FeSO4 x 7H2O 500 mg

MnSO4 x H2O 154 mg

CuSO4 x 5H2O 39,5 mg

CoCl2 x 6H2O 20,3 mg

Na2B4O7 x 10H2O 17,7 mg

Distilled water 1L

Dissolve the EDTA in 700 ml and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metals if necessary. Store at 4°C. Do not autoclave.